Variants of the industrially relevant protease KP-43 with suppressed activity under alkaline conditions developed using expanded genetic codes

In the present study, we attempted to control the pH profile of the catalytic activity of the industrially relevant alkaline protease KP-43, by incorporating 3-nitro-l-tyrosine and 3-chloro-l-tyrosine at and near the catalytic site.Thirty KP-43 variants Sega Saturn containing these non-natural amino acids at the specific positions were synthesized

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Performance and Stability Margins Comparison of Pole Placement and Optimal Controllers using Cart-Inverted Pendulum System

In this paper, pole placement and two optimal control techniques which are the linear quadratic regulator and linear quadratic Gaussian are compared.A cart and inverted pendulum which is an inherently unstable dynamical system is used as a case Sega Saturn study to analyze their performance and stability margins.Lagrangian equations defining the sy

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